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KMID : 1177720210150010065
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Journal of Alternatives to Animal Experiments
2021 Volume.15 No. 1 p.65 ~ p.72
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Role of Intestinal Microbiota in Crocin Metabolism and Carbon Tetrachloride-induced Lipid Peroxidation in vitro
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Shakya Rajina
Jeong Tae-Cheon
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Abstract
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Role of intestinal microbiota in drug metabolism has not widely been considered yet, despite of its importance in the biotransformation of xenobiotics. Crocin and its aglycone, crocetin, originally isolated from the fruit of Gardenia jasminoides, are used in treatment of inflammation, cancer, metabolic disorders, cardiovascular and neurological diseases. In this study, in vitro metabolism of crocin to crocetin by intestinal contents from antibiotic-treated rats were investigated by using HPLC. As consistent with the in vivo results reported previously, in vitro results also showed better metabolism of crocin to crocetin in intestinal contents isolated from normal rats than those from antibiotic-treated rats. Moreover, anti-oxidant effects of crocin and crocetin were compared to specify which compound would be pharmacologically more active in rat liver S-9 fractions that contain metabolic activation system. The results showed protective effects by both compounds against CCl4-induced depletion of glutathione and/or elevation of malondialdehyde. Overall, the results suggested that intestinal microbiota might be responsible for pharmacological actions of crocin, and that the present in vitro metabolic activation system would be a useful tool for studying the role of xenobiotic metabolism in pharmacology and toxicology, which can reduce the number of animals used.
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KEYWORD
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intestinal microbiota, crocin, crocetin, in vitro, Liver S-9 fraction
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